Primer
Part:BBa_K2973016:Design
Designed by: Nick Delkis, Aphrodite Katsaouni Group: iGEM19_Thessaly (2019-09-28)
IS6110 Forward Primer with 5' Overhang
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The primer design was accomplished using the Primer3 software (Primer-BLAST). The T7 Promoter on the 5' end of the sequence allows for downstream transcription of the amplified product by a T7 RNA Polymerase.
Source
iGEM Part Registry NCBI Mycobacterium tuberculosis Genome, Strain H37Rv, Accession Number: NC_000962