Primer

Part:BBa_K2973016:Design

Designed by: Nick Delkis, Aphrodite Katsaouni   Group: iGEM19_Thessaly   (2019-09-28)


IS6110 Forward Primer with 5' Overhang


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The primer design was accomplished using the Primer3 software (Primer-BLAST). The T7 Promoter on the 5' end of the sequence allows for downstream transcription of the amplified product by a T7 RNA Polymerase.


Source

iGEM Part Registry NCBI Mycobacterium tuberculosis Genome, Strain H37Rv, Accession Number: NC_000962

References

https://www.ncbi.nlm.nih.gov/tools/primer-blast/